Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDYSKFQTTTDRSDMFYWQGDRPFDPIETKEIFLDRHETYDENVAKAAVSYGMQQAGKSETD-A-----EVVHIDPSIVVGSVNIVCRAVLKD--------ETAVIIRMHPQKIHNGYFWSESVASQIAKDNGVPTYTTYFVDDSQSKFDFDYMIMECVPGKNIRKDMWPLSKELDKKIAQETGYWAAKINSIATSGYGFFDNAKAKKEKKLAGVHTKWEEHLFAANEKNIGYLEDKQVITNEERKTIDAIFDTKKSYI--NCENPKLIQNDIADWNELASDD-----GTITGVLDWDECFSGDPIMEIAAWSLFFG--------------------GERLENFFIGYRKVIEA-------------EGGFEEKYHIYRLRYVLSKLAMRKKKTEFTYYDTQFQRSLVNVGLEALREELTWQSTHH 5FTG Chain:A ((8-374)) ----------------------------------------TRRRAYLWCKEFL---P----GAWRGLREDEFHIS-VIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----EAMVLESVMFAILAERSLG-PKLYGIFP-------QGRLEQFIPSRRLDTEE-----LSLPDISAEIAEKMATFHGMKMPFNKEPKWL--------FGTMEKYLKEVLRIKFTEESRIK---KLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDY-AQARFDAYFHQKR------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1091 7522 6.89 26.12 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 6.89 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: