Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYPGINETQLELIFSRYG-K--DCKIEPLQEEASTRRYFRITSVNGKEEVVCADET-----VNEDFIVISEFLNA-NGIRVPRIHDTNQNLGLTFMSFEGLKDFSTYNLN---------DYKNKFPILIDLILKLQSLD-PP-SLVKTRKFDTEKLSFETNLTLDKFEAFRKQFQI-KTDITNEAKAFIEETVGYL--NKYPINVFTHRDFHCRNILIAPNTDYALIDFQDARMGVPQYDLASILYDAYYPLPRDFRSLMLKSFRERNVDQTKKFNDTFYLQALQRSFKALGTYFRMVTDHKKDKFKTSIISCLNQLEEIIQLGMFADSLYIFVRSLREELSRHKEFKNL 5UXD Chain:A ((5-257)) ----EDLDALLDLAARHGLDLDGGTLRTEEIGLDF-RVAFARAHDGGDWVLRLPRRPDVLERAAVEGRLLAMLAPHLDVAVPDWRIS--TSELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEVRSPAQV---RGAWRQDLARV---GAEFEIAPAL--RERWEAWLADDGCWPGHS-VLTHGELYPAHTLVEDERITAVLDWTTAAVGDPAKDLMFHQVSA----PSAIFEVALQAYAEGGGRPWP-----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 909 13810 15.19 60.04 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 15.19 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.359

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: