Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRSVHDALASHPVET-TVHRRLHDVRPHEVFEVTYD-GQRVVCKVAQHP--LGDPELEARILQFVAAETPVPVPPIMAVGS---------DHFVAGWCRDLPADP----TVDETRSRAMGAGLARLHESASERFDAPGHLEAENGSLALDADDRWSDTLVAFLDERRRFLEPRGYGDVATEVLGLVEAFPEAFDDVGDPTLLHGNALPDHVGIDRSADPARVTRVIDFEHALVGPPAFDLLRSIGPTYGPPGATHDPDGRAAFLEGYATVRPLP-ADLEETFRPLGVVNGVAYLRALHLQRGDRDAPQSVARRARGLAEHVLEAASEVRSALRSE 5IGH Chain:A ((10-280)) --SQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIR--------TPTQARQKVADDVDRVRREFVV--NDKRLHRWQRWLDDDS---SWPDFSVVVHGDLYVGHVLIDNTE---RVSGMIDWSEARVDDPAIDMAAHLMVFG--------EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALF---------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1056 -15848 -15.01 -62.64 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -15.01 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.313

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: