Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGDYSKLHPKTAQRILNQYGIE-DEFTLKALSLGISNSNYKVDTGNKQYLLKVSNDKG--FAELC--DEQHILKHLKDLGFPYSICALPTIKGELVYKQEDLFGVLFPFIKGIPPGP--SDITCRE-IGRALGKLHATTKDKE-LDVR-EYA---AIGQDAKTIREFID---D--P-SC---PADFSEAFHQVFSDDLKGYLSVNKTRCLIHGDLYYDNTLFWD--ESIAVLLDFEQAGLGNALLDLGISISGSCIEK---------GQINPQLVESFCEGYKL--SHALNDNEKNYLADAILLGLFSIALWRIKRFKHGNLNPMLENSYRDLLNKAKSFKTIIDSKGLKFEL 4OCV Chain:A ((24-334)) -------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLD-IVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYL-SHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMRFLADYLEG---------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1198 16565 13.83 60.02 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 13.83 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: