Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PEHCFVRILKYEIPIHGDGYLVG-SDVN--WWVGNQKVDFHINNDCTIQRRGGTQEKK-------------- 1BF4 Chain:A ((1-63)) ------ATVKFK--YKGEEKEVDISKIKKVWRVG-KMISFTYDEGGGKTGRGAVSEKDAPKELLQMLEKQKK

General information: TITO was launched using: RESULT: Template: 1BF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 147 9080 61.77 197.39 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 61.77 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.123

(partial model without unconserved sides chains): PDB file : Tito_1BF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1bf4-query.scw PDB file : Tito_Scwrl_1BF4.pdb: