Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGVMRSPLLLAALADAAVPGIRPIKVEGLPVPAGDLYQSAHITGA-DGRVWLVRSALTGAAGAELAASDALVQLLTRR-VDFDVPRVEGSARPKEGSAVAVYARPRGRALDWRHL------------AGRSAAARALGQALAHLHGVDPRVVEEAGLATYDADTYRQRLRADVDRAAATSRVPSALIARWREALDTPALWRFSTTVTHGPLRGADVHVDE-GEVSAISGWEHAGVADPARDFALLWNSAPQQAFDTVFEAYAAERAERPDPNLERRVRLMGEMELAYALLASRTMGDDARVERHAAALEELAFEVEGDSSLLPPARRAGTAPIDLVPPLDPADIEVVDAEVGEEDATVEIPVRLAPEREDRSEHAGATRVDGDAARTRLPHGSSEEPAASSDDSAGAVADESSGGNARSR 5IWU Chain:A ((1-297)) --MSKDIKQVIEIAKKHNLFLKEETIQFN--ESGLDF-QAVFAQDNNGIDWVLRLPRREDVMPRTKVEKQALDLVNKYAISFQAPNWIIYT-----EELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHTPEEARMSMKQRMDAVRAKFGVGENLWNRWQAWLNDDDMWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAFGEEGLEALILAYKEIGGY-YWPKMKEHIIELNAAYPVSIAEFALVSGIEEYEQMAKEAL----------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IWU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1269 5822 4.59 20.64 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 4.59 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_5IWU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iwu-query.scw PDB file : Tito_Scwrl_5IWU.pdb: