Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVKSPDLPVLPGSDQVAPASAIWSSETWRWEAVRWMDTALARRGLERVPTTPLQPRVRPWSTVLVAETTDQ-GRVWFKATSP-----EMSPEAAILRALRSVTPDAVPVVWADDPE-------RGWLLMPD-QGPVLRDVQRDDDAVSLMSSVVRRYARLQRSSTRVVDQLR-DAGVPDMSPR----ESARRWQRL----GLKPDTTREVRRAAQRLDAVGLPLTIQHDDLHAGNVFADGTSAGAHDARIFDWGDASVGNPLCSLLVPLERIGEGLDDDAARAARERILRAYLSVWSDVVSSTALSAAVDDALLIARVGRVFTWQRALTRASADERHAWGVHGRRLIAEINRSLGHAPEPSHD 5IGI Chain:A ((12-239)) -----------------------------------LYALAARHGLKLHGP-LTVNELGLDYRIVIAT-VDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV----VPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNT---ERVSGMIDWSEARVDDPAIDMAAHLMVF-------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 705 3423 4.86 16.78 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 4.86 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.264

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: