Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRRHMNRFTIEQVLDIAEVIHSRGSRKVWRIG------DYILKQTPYE---GVNNEVATLRFVQQHTTIPVPVVYDEWLSPDRRFHYILESRIPGKSLKQCWGVLSRKGRERIARTVLKYMQELSQFRSDRLESISSNKLRLNNFVPKPYKVLLNIWRTDNDIFDNEYRPA---LL-SSGVDDGVILVLKNTMPPCQGQYTLTHGDLFTGNIIVDPVKEQVTGIIDWESAGFWPAWFQYARITLGAGGWDDEWKALLSRAMKATHLPPHSKHGRIWWDAVYELLDHPGSSLAREWIKEIIRYLAGENVSLNNYDGFGL 4GKI Chain:C ((31-254)) ------------------RDNVGQSGATIYRLYGKPNAPELFLKHGKGSVANDVTDEMVRLNWLTA--FMPLPTIKHFIRTPD--DAWLLTTAIPGKTAFQVLEEYP-DSGENIVDALAVFLRRLHSIPVCNCPFNSDRV--F------------RLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPF-SPDSVVTHGDFSLDNLIFDEG--KLIGCIDVGRVGIADRYQDLAILWNCLGEFS---PSLQKRLF-QKYGI---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4GKI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 841 26824 31.90 127.13 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : 31.90 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.248

(partial model without unconserved sides chains): PDB file : Tito_4GKI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4gki-query.scw PDB file : Tito_Scwrl_4GKI.pdb: