Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRPVPADLLEITDALLPGAPLDSARLAEHGNMHHVVLLP----GIAAVRVSKRAGAAAELPRRVGILHAVAAAGMPFAVPEPMTAVTTFGER----AAVAVSWIDGASLPEG------TGDPTVFGPLLDALREVRISPELEAALHRPRRRADDGSGWADILNNQVIPRLPARWRDRVRQRSDELLAI---EEVPASLVHGDLGGGNVHFGADGTLTGVLDWDMAILSDPAIDAALVATWHGWHMLRAAT----------DEQIYRRAGAWDAAVGAGHLHAILRGRPLSNVDGFVRSVIAWLEDPGRCRDR 5IGI Chain:A ((6-281)) --TADTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNR-LPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQA-RQKVADDVDRV-RREFV--VNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFA-----------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1059 -41912 -39.58 -169.00 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -39.58 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: