Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHPTPSPDTILFASHTTATAASVAQWVERHYDLPVQQCYLIRRNLNDNYAVRAADGSRYVARLCAIRPRGPFNVDFEVALLAHLEAQGVGVASPVLAANGATSVALPFPEGPRVLVLFRHCEGSVPDTP--EDLHLTGATLAQIHSAAQSYTGPASRYVLDGHHLAGRTLDYLAAHPELAPSVLDAYRELIHRLLGELANV-EDTLTRVMCHGDTHGFNNHVYSDAEGARRAAFFDFDDAGPGFLAYDLCVWLWSNLPRKAPMEPDEALLQRWQQYLAGYRAGGGHVTDADLAALPLFVQLRHLWNMGEGVARIHHWGANMMSVDWIKKQLEVMAAWARLDLPA-------------- 1ZYL Chain:A ((4-328)) ---SAFT-------FQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQT-----LLNHQGFYFAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQL-FIHRPTIGLNEYLIEPRKLFEDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGN------ILWRDGPMFVDLDDARNGPAVQDLWMLLNGD--KAEQRMQLETIIEAYEEFSE--------FDTAEIGLIEPLRAMRLVYYLAW-LMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1432 -68161 -47.60 -221.30 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -47.60 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: