Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPFDEVRRTVTGHGLELVRAHPRRDRLHLDLRGPDRQRVIGQWIDDATEATRVADATPGRVERLGSRLVLQHDGADRRLPALADLVAGGAELVVHRPERRAVVRYQRG--DGYVYTKVVRPSRT--AELVRRVRAAA--AVPGLSAPEVTQWDEAGGTIVLTTLPGQTLHDLLTGVVPAVVATEVGRAVRTLHSAATAPDGTTTH---------DLAAEVDATRALLDLARTHRAL-----------QPREALVVDRRIAAAAATIAALARPAAPSLLHRDLHDKQLLVDG--ASIGMLDVDTLGLGDPALDLGNLLAHLDLRVRQGWTQRETAAAVEQGVLDGYVPD-DRT-----RAATAGYRALAAARLEALYAFRPGDLPGGGQPAK 2QG7 Chain:E ((123-429)) -----------------------------------------------------------------------------------------------------ILIKVKDMSKQAKYLIRLYGPKTDEIINREREKKISCILYNKNIA-KKIYVFFT--NGRIEEFMDGYALSREDIKN--PKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVE--VESLCKRENSPIVLCHCDLLSSNIINTVGGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCD--WDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQ------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 893 6318 7.08 26.77 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain E : 0.62 3D Compatibility (PKB) : 7.08 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: