Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHVPLSIHTTSYSMAAWDITTADEAWLIELCHKSADEGRQIGGQEHGGPKVVRIS-----DRIVAKYGNIRRSELAAQEVAYHNTDRSIVHIPKVHRFFESDGQSYLFMEYVEGQTLEDVDFE-------THKDIPIRVANILAHLQQILGDCDSPGPVTGGEAHGYIFGDEGAGTAFDSIEDMNVYMNKRLDKMNEYLSRQEDQRRFDHLDLTPYALVLCHGDICRRNIIFESDG----SLCLVDWGFAGFYPRIFELAAISYVVQNNAAFKDPIIHEFTKLLSLTDKEKQDIDRFRAVRFANLRWSFRDRRTTAEEDKFIQELYETQKRIKEEQE-----TAGIQSFIISELLDGLL--EQKSRPDLEVRHVPRPWTGSQTAIGHVKRVVVVGLDVNVASFILAMKILSSRRIFVIRTTQGSPK 4NIF Chain:A ((16-270)) ------------------------------------------IGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYG-QHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLH---------------------------------------------------------------------SQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCY------FVAPEVLK-----RQGYDEGCDIWSLGI-----LLYTMLAGYTPFANGPSDT-PEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQV-LQHPWVTQK--------------------------------------------

General information: TITO was launched using: RESULT: Template: 4NIF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 966 21709 22.47 94.80 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 22.47 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.183

(partial model without unconserved sides chains): PDB file : Tito_4NIF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4nif-query.scw PDB file : Tito_Scwrl_4NIF.pdb: