Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------MSAENLSQMGAANVQRLVDQYGQQVIEKCPVSPVEYAFYERAAEELNSRGIKTPTLLSADPHPRRL----RLEYIPGKVSQDEVS-GDDILKCLSHLHHTPPDPTWVYHPHSWPEKALEKSLALLR-LPDRAAHQMRCFQQAGS-VLFRQKC---LISGDTN----AGNWGRRENGEW-----VLFDWER----------FGTGSP---------AIDL----APLVKG-MG---SRQSILQLAE-RYS-VHCP-----------HMPARQLATEITLAKAWIVSEVMSLLYD--RNKTDFELYLNWYRETLPGWLDEAISVL----------- 1IQ8 Chain:A ((6-360)) KMLKFEIKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQMVVEPKELEKMGFEII--ITNSY---IIYKDE-ELRRKALELGIHRMLDYNG---IIEV--DSGSFQLMKYGSIEVSNREIIEFQHRIGVDIGTFLDIP--TPPDAPREQ-----AVKELEITLSRAREAEEIKEIPMNATIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLESYRFRDVVDIVISSKMALRPDRPVHLFGAGHPIVFALAVAMGVDLFDSASYALYAKDDRYMTPEGTKRLDELDYFPCSCPVCSKYTPQELREMPKEERTRLLALHNLWVIKEEIKRVKQAIKEGELWRLVDERARSH--PKLYSAYKRLLEHYTFLEEFE

General information: TITO was launched using: RESULT: Template: 1IQ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1348 24354 18.07 100.64 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 18.07 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.236

(partial model without unconserved sides chains): PDB file : Tito_1IQ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1iq8-query.scw PDB file : Tito_Scwrl_1IQ8.pdb: