Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNQNVLLNISG----VKFVLRIPNAVNLSLINREYEAFNNAQAYRAGLNVETPVLDAKSGVKLTRYLENSNTLSQIQLNEQSCLSQVVNNLYRLHNSEFVFRNVF-------SVFDEFRQYFSLLENKSA---FYQA---DSR---MDKLSAVFWQ---------FEE-I-NKEVILRPCHNDLVPENMLLQDDRLFFIDWEYSGLNDPLFDIATIIEEAHLS------------------KEAADFLLETYCNQTNKYH----KTEFQIAHKRLKIHRFCQNVLWFLWTKVKEEH-GENFGDYALKRLDAAFKLLEELP 3MES Chain:A ((83-408)) -TNQLFVVSIVNELKHPRILFRIYGKHV-KFYDSKVELDVFRYLSNINIAPNIIADF--PEGRIEEFIDGE-PLTTKQLQLTHICVEVAKNMGSLHIINSKRADFPSRFDKEPILFKRIYLWREEAKIQVSKNN-IDKELYSKILEEIDQLEELIMGGEKFSMERALELKLYSPAFSLVFAHNDLQENNLLQTQNNIRMIDYEYSAINFAGADIANYFCEYIYDYCSEKQPYFKFKYEDYPCEELRKLFISVYLSQTLQEQVMPSQQIVHIMTKAVEVFTLISHITWGLWSIA----VEFDFTEYANTRFTHYLQKKKE--

General information: TITO was launched using: RESULT: Template: 3MES.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 949 1547 1.63 6.07 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 1.63 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_3MES.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3mes-query.scw PDB file : Tito_Scwrl_3MES.pdb: