Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAPFSIKQADIQQQLTAAIQKHFPA-VEI---ADIRFELV-GGLSSKSWRIRGPG---------IEWLARRQSPTERKM-GVDRQREFALLRRMSAIGLAPRPLLWRDDWLIVEWVPGRIATSDEFLMMLANGEVARVLSQLHRLPRSGHPL--DIKMLFAQHWQLMDPHRR-------------SPVLLRAHHYFQRAPLPTPLALAPLHLDVHAENLLITPQ-------GTMLIDWEYASDGDIAFELAFIVRASQM------------------DSMAQRYFLQSYQRHRRGF-----------SVNRLQQQMRQWFPWVDYLVLMWFEIRWQQT-KNPEFLADIPALYHRLQLGHRLQPD 5FUT Chain:A ((8-371)) ----------TRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG------GAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFH-------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1043 -52673 -50.50 -192.24 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -50.50 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: