Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAFDVLARLGVDPPDLELLRFG-ENALYAFRE---------R-R-LVVRVARPSTDPTHVAQTVEFVRRVAARGMPVSEPADLTELVQPVVTSTGVVTLWTLHDVDEGC------RVSAAELGGLVRQFHELAAPEADLLDE-WEPFGLIRSRLAAAERD----GFDRS------LTGPLVDLLASLETAVDRLSARLGIGVMHGDMHYGNVLCLPGRRLLLIDFDQVCRGPREWDLVPNLVTVRR-FG--------------LAESDYQEFCAAYGFDL-------------RTSPDVETLVRLRELGMVSWLLQQYGTSA---VIDDEIRLRVGTIGESVGPPATRWWAH 1NW1 Chain:B ((75-415)) -------------HLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPETESHLVAESVIFTLLSERHLG-PKLYGI-F---------SGGRLEEYIPSRPLSCHEISLAHMSTKIAKRVAKVHQLEVPIWKEPDYLCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHISL--SKSPVTFCHNDLQEGNILLPK--RLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVELSPVGFGFADYGRDRLSLY--------------

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 983 15444 15.71 61.53 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 15.71 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.212

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: