Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADTPPLTLPEALRRTLPAARWELTHTNDAGTRVYRSQ----RFLIKVHPRGPSHPLFSEKERLRWLAPR-VPVPPLAGYAEDADHAYLALARLPGMPMNHPDARLHARRNADLLARALAELHALPIRDCPFTHTLTERLRDLRTRLTAS-------GE--IPTPVAERFNALVRQRPHDEDLVVTHGHATLEHVLVNGE--YVEALTGVGRAGLADRHVDLAAAHTSLTQD----YDPDAAAHFLDTYGRA---RIDLHKLDYYARLNALA 4H05 Chain:B ((13-266)) -----------------PGCEWVVVEDGASGAGVYRLRGGGRELFVKVAALGAGVGLLGEAERLVWLAEVGIPVPRVVEGGGDERVAWLVTEAVPGRPASARWPREQRLDVAVALAGLARSLHALDWERCPFDRSLAVTVPQAARAVAEGSVDLEDLDEERKGWSGERLLAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLRELAHEEDPWFGPECSAAFLREYGRGWDGAVSEEKLAFYRLLDEF-

General information: TITO was launched using: RESULT: Template: 4H05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1033 -13241 -12.82 -57.32 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -12.82 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_4H05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4h05-query.scw PDB file : Tito_Scwrl_4H05.pdb: