Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------MEDVQAVRGYLERSGWLE-GIREVRF--LAAGEYNENHYVRASGGE---------YVLRINHGSQLGLGREQIGYEFSVLSAVAPSGVTPRPYRVDPWPEGLSGGTLLMDYLPGRPLD-----YSHDLERAARILARVHALPPDDGLIRQDRAVLDIVAESEGLLDRY--PDHP--RPEMRDRLLAYRDDVLTLNERSRDFLAS--EPPVMVNTEVNSGNFIMSHMDGRHGKGDFLVDWEKAVVSTRHQDLGHFLAPTTTLWKTDT----------QLDREQREAFLSAYRRELLLLGAAAPDMDALREGSAILERT---ILLRALSWSHMAYCEYTRAERAIR-NADTLAKIRQYLDNAECFLTWKE 1NW1 Chain:A ((32-426)) GMKELLSTMDLDTDANTIPELKERAHMLCARFL-GGAWKTVPLEHLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPE---TESHLVAESVIFTLLSERHLGPKLYGIFS-------GGRLEEYIPSRPLSCHEISLAHMSTKIAKRVAKVHQL--EVPIWKEPDYLCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHISLSKSPVTFCHNDLQEGNILLP-------KRLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQ----GNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVELSPVGFGFADYGRDRLSLYFKHKQLLKNLA-

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1282 22679 17.69 74.11 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 17.69 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.376

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: