Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METKLGRKIGEGGCSEVYEYGTE--QPVKIIKLAKSNT---SMDAMRIEYRNHRTTWELGLPVPQPFDLRVVDGRPGIVVEQIQGETLMQRFLRGLDQPEVSEDSPIYDDVRMTARILNALHSHSAEALPSQRESIKGALLGVESLTAPEKKEICAILDDLPVKQRLCHGDPNPGNIFVRHDE-GLLIDWMNASAGNPEADLAEYILMVRYAVLPSHLGARIIGRFHSIREMMIDAFMEEYTRLSGVTAEEVDPWMLPMAARKLSADAISDEEKGLLLHEIRVRLNAYKGAEESFRE 5TVT Chain:A ((11-210)) -YYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSDLPRIKTEIEALKNLR--HQHICQLYHVLETANKIFMVLEYCPGGELFDYIISQD----RLSEEETRVVFRQIVSAVAYVHSQ-----------------------------------------GYAHRDLKPENLLFDEYHKLKLIDFGLCAK-------------GSLAYAAPELIQ--GKSYLGSEADVWSMGILLYVLMCGFLPF-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 5TVT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 843 -38372 -45.52 -212.00 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -45.52 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.138

(partial model without unconserved sides chains): PDB file : Tito_5TVT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5tvt-query.scw PDB file : Tito_Scwrl_5TVT.pdb: