Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKVEAILREMGDQSALNEVTPVSGGDISRAFQAKTA-NGVYFIKINDKAP-EDFFLKEAEGLNLLRQAGALPVPQVYYVSPPSAEDGY----IIMEWVKG-ES--------APNTESRLGHGLALLHQHTHTHYGLAENNYIGRLPQPNRWEKSWTRFLREHRLGFQAKLAEKRGRLPAGRKTRLEKLLDRLEQWVPDYRQPVLLHGDLWSGNWLAGP-RGEPYLIDPA-VFYGEREFELAFTELFGG--FSPRFYAAYRDVQPL-S----DNYEEVRPLYQLYYLLVHLNLFGESYGPSVDRILTYYAG 5IGH Chain:A ((10-299)) --SQLYALAARHGLK-LHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGM--LI--RTPTQARQKVADDV-----DRVRREFV----VN---DKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLAA---

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1179 -26184 -22.21 -98.44 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -22.21 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.302

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: