Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKPELTATSVEKFLIEKF-DSVSDLMQLSEGEESRAFSFDVG-GRGYVLRVNSCA---DGFYKDRYVYRHFAS-AALPIPEVLDIGEFSESLT----YCISRRAQGVTLQDLP-ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVA-QALDELMLWAEDCPEVRHLVHADFGSNNVLTDN-GRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAG--SPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE 5IGI Chain:A ((5-288)) ---TTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIR---T-PTQARQKVADD----VDRVRREFV--VNDKRLHRWQRWLDDDS-SWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGE-EGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEE---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1225 -20067 -16.38 -74.88 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -16.38 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.292

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: