Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFARDALSTAVETACDPDPAVDTISTIEAGNN-HTYRVTLADGTDLFLKVGTRF--PDAFPAEPATMRRVRAETDLPVPRVHGTGTDPLEYPY----AVYEYVPDCEAEWAGDLSHEAAERLCREAGRHLCELHQITFPQFGRIGLDTDASADDTLVADAPDADALTVVDPTPFDDLLRQSLDRQVTELADSPFDTRRDSLEAFGTDLIDRSDFADVTPALVHGDYRLGNLCVDPSAEQITAAVLDWELPTALDPLWDAVMAQSLLTAGHRLDPDSRQTLRRAFWEAYGTSTADTPRRRCYELLARIRLARHLDTECRGLSEAARRARITDHHEAFDALLVDR 5IGI Chain:A ((7-256)) --ADTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV--VPQDSEVFAESFATALAALHAVPISAAVDAGMLIR--------------------TPTQARQKVADDVDRVRREFV--VNDKRLHRWQRWLDDDS----SWPDFSVVVHGDLYVGHVLIDN--TERVSGMIDWSEARVDDPAIDMAAHLMVF----------GEEGLAKLLLTY-EAAGG-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1019 -4875 -4.78 -20.14 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -4.78 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.254

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: