Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFGNIKRTKQLKSIIRQHNKS--IKYVKQINRGYYGYVFLITDPFGKKSVAKVYKKD----DYAKREMAQLEMLAKHSLAHIPEIYAIDTKKQ--------NGFFDILFMEFIDGVDASRIKVIDEKEKMRLSNEIVDNLIAIHSVSNPNGFGDFVSGNYFERWQDYYKLQIDSLFGSLKSDRP-KKFSKQSFSIAEKLYYSFDDVFCLEVKQNSLIHGDYNLWNLMVDPAT-NRLAAVIDPFGCSFADRELDLFQLQNSN-------GNEYRLLENYASKIPLSEIFEIKNAYYRFWDDIKHLVNVGYCDNDSFCKYGNMTLELLNR 6EF6 Chain:A ((27-314)) -------DVTIAQQALTHYDVSDNASLRLL-NLSENATYLVEDG-EHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPD-EES----LTLDDFQTLGRITASLHEHSQRWTRPAGFGRFS-WDWEHCLG-DTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEY-GSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLL-------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1174 31773 27.06 121.27 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 27.06 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: