Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQIVEDAGSLDVFRWPSSSPPSPENLEHAARLCQGALGSTSVAVVATPYNHAGWSAQDCIKVSFPSENRSFIAKVPRLVDENSATACKNEAQRASWASQHQIGPAVIAIDDLSGAFAMEFLSGQ-TLSAQMMLQDHLKTCTIGLLRRIHDAPSQHY--MQIYNAPAAVKRMLQAADKERLLDGDDGLLLESLITWTFQKVGCQPTFQTLVPCHNDFHSQNIMLDQQGTLWAIDFEDCDLGDPMWDLGYLTANMGLEPL--D-LAGIY-GCNMEQ-KKRLEVFYYLAIGHCAVWSAIHGPLWTQHYRDCMLRLRRGWLRL 4R7B Chain:A ((18-297)) ----------------------GSHMEKIIKEKISSLLSQEEEVLSVEQL--GGMT-NQNYLAKT--TNKQYIVKFFGKGTEKLI-NRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTS--IKTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQI-PY---ANYESVRNAVFSLEKRLAD---LGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRAV---

General information: TITO was launched using: RESULT: Template: 4R7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1262 -29524 -23.39 -108.54 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -23.39 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_4R7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r7b-query.scw PDB file : Tito_Scwrl_4R7B.pdb: