Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESTDEPGDTVDTELFTRVVRHGLPDVTVETVTPGGPSWNPVTEVARVGFADRDPVYCKAAPGDH-EGDLRAEAGTLDYVGAT--LRVRVPSVVRVT-TEPVAALLTEPVAGRAVAD-EWFETTPERRATLAERLGRTLATVHTERFDRAGEITGGDPDGLALDHAPWPEVLRAGVTETQRRAPTDRFDPECARLLDAIDDSRDRLADPPARLLHRDPATPNCFDTGD-DRLTLLDWGNSVVGDPVCDLVRAREQVLAPSREPAPDRLVAPLRRGYRAVAGGVPSAADREPVYEAAITLSTAGFVDRLAEWREESVAELTAWFRAELDGRLSAVE 5IQI Chain:C ((9-281)) ---------ATNVKAMKYLIEHYFDNFKVDSIEII--GSGYDSVAYLVN----NEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTI------DNKQNVLEE-YILLRETIYNDLT-DIEKDYIESFMERLNATT--VFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEI----GTNFGEDILRMYGNID--IEKAKEYQDIVEEYYPIETIVY-------------------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1164 5785 4.97 21.67 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : 4.97 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.299

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: