Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------CVIG--LTF-NGNWV-PNNRFVISPG----GKFSFIANYVII------PVRLDESCHWHQDLAENA--KPVIIPSGYGIKSC------------------------------------------------------------------------- 1V3W Chain:A ((1-173)) MAIYEINGKKPRIHPSAFVDENAVVIGDVVLEEKTSVWPSAVLRGDIEQIYVGKYSNVQDNVSIHTSHGYPTEIGEYVT----IGHNAMVHGAKVGNYVIIGISSVILDGAKIGDHVIIGAGAVVPPNKEIPDYSLVLGVPGKVVRQLTEEEIEWTKKNAEIYVELAEKHIKGRKRI

General information: TITO was launched using: RESULT: Template: 1V3W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 308 -13623 -44.23 -219.72 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -44.23 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_1V3W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1v3w-query.scw PDB file : Tito_Scwrl_1V3W.pdb: