Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAVNRLLSECLGPAEIRERTELPGGDIHEAWRLSYG-EKEVFVKCNTREM---LPIFTAESDQLSLLARSKTVQVPEVYGVGSDRDYSFLLLEYIPLKPLDAHNAYCLGQQLAHLHQWSEQLQFGLDFDNDLATTPQPNSWQRRWAQFFAEKRIGWQLQLAAEKGMSFGDIDDITNAVQERLQ---SHQPQPSLLHGDLWPANCAASSNGPVIFDPACYWGDRECDLSMLPLY-PTLPAQIYDGYQSVWPLPIGFIERQSIYQLYYLLNRSNLFGGKHLINAQKAVDNLLHPEQFSL 3F7W Chain:A ((2-287)) -VNSVAARVTELTGR-EVAAVAERGHSHRWHLYRVELADGTPLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQVAGWD----DRTLAMEWVDERPPTPEAAERFGHQLAAMHLAGAE-SFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAADRGALTPGDVRLVEKVLDALDHLAGDPEPPARIHGDLWNGNVLWQDDGAVVIDPAAHGGHREADLAMLALFGLPYLDRVRDAYNEVAPLAEGWRARIPLHQLHPLLVHVCLFGAAYRTTLVDTARAALR------

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1496 -49968 -33.40 -179.74 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -33.40 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: