Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQTLIAALQNPALYPHPVERFEVIETHISWVLLTGPYAYKIKKPMDFGFLDFTSLDKRRFYCNEELRLNQRLTDDLYLEVLPITGSEEAPRIGGDGEAIEYLLKMRQFPQDNLLAAIQGRGELSNQHIDELAEQIAHFHQNTPKVPLEHPLGTAESCMAPVRQNFEQVRPLLKDKADLQQLDALEAWAESSFERLLPVLQERKAKGFIRECHGDIHLGNAALIDGRVVLFDCIEFNEPFRFTDVCADFAFLAMDLEDRGLKCHARRFVSLYLEHTGDYQALELLNFYKAYRAMVRAKVALFSLGYQTDAVQRAATLRQYRNYANLAESYSAIPSRFLAVTHGVSAVGKSQVAMRLVEAL------GAVRLRSDIERKRLFGEQPEHDRGQFAAGIYSSQASEATYAHLHQLAAKILQAGYPVVIDAAYLKQNQRQAACHIAEETGVPFLILDCQAPEAVIAGW----LAQRQAEGKDPSDATLDVIRTQQATREPLTAAETVQSKRVDTHDAASLDGLVDGIRQRL--PGL---- 1I2D Chain:A ((391-573)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PRATQGFTIFLTGYMNSGKDAIARALQVTLNQQGGRSVSLLLGDTVRHELSSELG-----------FTREDRHTNIQRIAFVATELTRAGAAVIAAPIAPYEESRKFARDAVSQAG-SFFLVHVATPLEHCEQSDKRGIYAAARRGEIKGFTGVD------DPYETPEKAD----LVVDFSK-QSVRSIVHEIILVLESQGFLERQ

General information: TITO was launched using: RESULT: Template: 1I2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 848 3575 4.22 21.41 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 4.22 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.193

(partial model without unconserved sides chains): PDB file : Tito_1I2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1i2d-query.scw PDB file : Tito_Scwrl_1I2D.pdb: