Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSNNNNPLTRDEILSRYFPQ---YRPAVATSQGLSGGSCIIAHDTHRVVLRRHHDPDAPPAHFLRHYRALSQLP-AS-LAPRALFYT-PGWMAVEYLHGV-VNSA---LPDADELAALLYHLHQQPRFGW-RIALSPLLAQYWSCCDPA---RRTPFWLRRLKQLQKNGEPRPLRLAPLHMDVHGDNIVLTS-AGLRLIDWEYAGDGDIALELAAVWVEDER---QHRQLADAYAARARIDARQLWRQIRLWHPWVIMLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG 4R78 Chain:A ((19-281)) --SHMEKIIK--EKISSLLSQEEEVLSVEQL-GGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSIKTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYESDQ---TPVSHEKIAIYKILQDTIWSLWTVYKEEQ---------------------

General information: TITO was launched using: RESULT: Template: 4R78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1089 -10976 -10.08 -44.80 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -10.08 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.306

(partial model without unconserved sides chains): PDB file : Tito_4R78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r78-query.scw PDB file : Tito_Scwrl_4R78.pdb: