Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTTNPAAPAAPLAPPDPARIAKTVATKMPFRGDMTGLTPLAGDASNRRYFRIALKGTPSALILMQLADPEGFKKSEEAVSGASAQIAELPFTNVLAHLQRTGVTVPQLHYYD-------REAGLLYLEDFGDLTLAEACRDADHARLVALYRQAIDQLVLLQVKGTKPPAPGCIAFHRRFDVPLLMWEFDHFLEYGIVARLGQPMKPADDKAVRAEFQRIAELL-----AGQSQVFVHRDYHSRNLMVDGSRIGIIDFQDALMGPATYDLASLLKDAYIELDDAVVDGLVDQFLDGLAAHGQGWADRAAFRRLFDFTSIQRNLKAAGRFVYIDRVKHNPKFLADIPRVLGYVKRNLSKYPELATLRQHLAPYVAELQ 1ZYL Chain:A ((5-280)) ---------AFTFQTLHPDTIMDALFE-HGIR-VDSGLTPL-NSYENR-VYQFQDEDR-RRFVVKFYRPER--W----------TADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEAD--------NIDQMEAVGRYLGRMHQTGRKQLF-IHRP---TIGLNEYLIEPR---KLFED-A--TLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRD-GPMFVDLDDARNGPAVQDLWMLLNGDK-AEQRMQLETIIEAYEEFS------EFDTAEI-GLIEPLRAMRLVYYLAW-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1157 -51317 -44.35 -194.38 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -44.35 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.278

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: