Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDGSASSSSPPSLSQPINDTTPRRLLTLCSVGVARPFQRKRWSPSLLRL---SPWTCVKLGTF----RDLCEARTIQFIASNTSIPVPKVYCSFQRRDKRDGTTTTYIVMQRIRGQTLASAWPTLSASSRNKILLQLRHFVQVMRNLPWPKREYKISNVDGGSLWDCRLVSGVDRFGPFKDTLAFHLFLRNGLEKA-PPELPEVDEMIKLQARDWGPVVFTHGDLSSLNILVRGD--RIVGIVDWETAGWFPPYWEYSTACQVNPRN---TFWAEWIDHFLEPWPDALEMERVRQRWWGVI 4H05 Chain:B ((9-248)) --------------------------RGRYPGCEWVVVEDGASGAGVYRLRGGGRELFVKVAALGAGVGLLGEAERLVWLAE-VGIPVPRVVEGGGDER------VAWLVTEAVPGRPASARW---PREQRLDVAVALAGLARSLHALDWER----CPFDRSLAVTVPQA---------ARAVAEGSVDLEDLDEERKGWSGERLLAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLRELAHEEDPWFGPECSAAFLREYGRGW-------------

General information: TITO was launched using: RESULT: Template: 4H05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 985 -19995 -20.30 -88.08 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -20.30 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.268

(partial model without unconserved sides chains): PDB file : Tito_4H05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4h05-query.scw PDB file : Tito_Scwrl_4H05.pdb: