Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGILEYTSQLDIEFKKALGLIFKQELNLERIKQGEVNYSYKVTSEQ-DIPLYHARIFRYEGH--PDTEKLLWISGELKKRNIPQARILYREKSSINFPNGFMVSNWIEGKNGWQSIKDGAYSLKNFTLETAKILLKIHEIKVASFGWPYG------KGWGYYNTYQDRITGLP-NNDEFKK-LFN---N-DYLKRKYVLNIVSLLLKALEKIPF-PEQPILAHSDPTPDNIIVRN--DRSLVLIDWDDSEGSWWVRDYSYLRY-WS--------DKPEEIKAGFFSGYGKIDMNE--SEIEIAELVEWILQSLRLLPYYKFSFADEGKYQKRMTRLKENVRQLKLLV 2QG7 Chain:E ((110-450)) ------------------------SLEFQIING-ITNILIKVKDMSKQA-KYLIRLYGPKTDEIINREREKKISCILYNKNIA-KKIYVFFT----N---GRIEEFMDGYALSRED----IKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWN-TIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGG-DSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSS-DFDFINYGMTRLTASC---

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1182 15528 13.14 56.67 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain E : 0.60 3D Compatibility (PKB) : 13.14 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.288

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: