Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVEQNEFLSSVQLQILAEQAIKQYPNEYQGSVKLLCQSENATFLVTTAQAKYAL-RIHRPNYHHLQDIESELAWL-DALNQSGIEVPLAIADQDGQRVITLKLAEGISRYAVLFNWVNGSMPTTDV-DPTAFQQLGQITAALHQHSKSWKRPEQFNRIVWNHDTMVGAQGHWGNWKHAPHLDQADHAVVEEAIARISTELCQYGAGEDRYGLI-----HADLRLTNLLLQD---QQIGVIDFDDCGMSWFMHDLAAAISF-------NEHLDAAPQWVEHWLTGYEKTGHVQNHDYVMIPTFIMQRRIQMMAWNGSHAHTEMALSLGDQWSNETVRLCKKYLNGEMPVGRSNA------------------------ 4OCV Chain:A ((24-378)) ----TNEALFDVASHFALEGTVDSIEPYGDGHI-------NTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEID--GGAWR---VYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHD-TPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDS------ITAREAELLPFSGNLLTMECGMRFLADYLEGDVYFATKYPEHNLVRSRTQIKLVREMEQRADETRAIVADVMETT

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1499 -19524 -13.02 -62.38 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -13.02 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.254

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: