Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFGNIKRTKQLKSIIRQHNKSIKYVKQINRGYYGYVFLITDPFGKKSVAKVYKKDDYAKREMAQLEMLAKHSLAHIPEIYAIDTKKQNGFFDILFMEFIDGVDASRIKVIDEKEKMRLSNEIVDNLIAIHSVSNPN--GFGDFVS--GNYFERWQDYYKLQIDSLFGSLKSDRPKKFSKQSFSIAEKLYYSFDDVFCLEVKQNSLIHGDYNLWNLMVDPATNRLAAVIDPFGCSFADRELDLFQLQNSNG---NEYRLLENYASKIPLSEIFEIKNAYYRFWDDIKHLVNVGYCDNDSFCKYGNMTLELLNR 3W0O Chain:A ((9-275)) --------TSVEKFLIEKFGSVSDLMQLSEGEESRAFSFDVG-GRGYVLRVNSCADGFYKDRYVYRHFAS-AALPIPEVLDIGEFS--ESLTYCISRRAQGVTLQ-D--LPETELPAVLQPVAEVMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA----EDCPEVRHLVHADFGSNNVLTDN--GRITAVIDWSEAMFGDPLYEVANIFFWRPWLACMEQQARYFERRHPELA-GSPRLRAYMLRIGLDQLY-----------------------

General information: TITO was launched using: RESULT: Template: 3W0O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1178 -75783 -64.33 -291.47 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -64.33 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.355

(partial model without unconserved sides chains): PDB file : Tito_3W0O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3w0o-query.scw PDB file : Tito_Scwrl_3W0O.pdb: