Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGGNMAEKDLATRVARVLGR-------NSVRLEDLPPGSSRGAARVLSEE------G-EPIAFLRIDLGVATDVTVGKALVSEMALVKRAHDLGFPVPKVFALLNDPPVAVMELAAGTARPDE-AEAERVGPEFMGHIARLHSIAPAEFGLDAPATVSDAIRQDLDCYMADAEARG-----------ITRRPLVRLAARILRHALPRSAEPPAMLHGDVGAGNFMVDNG------RVSAILDWELAHAGDIHEDLAWLWTRGAHT-----------------AFGDPRQRIAEYEAAAGKA------------L--DPDRLAWHVAFVTFKSVIGLQRRMYAQGDDPGLLIILIAILTYETLLCAALGRILGIQLDMMEEAPVEGVTETVRLIELMRHVAPPANREGELLQQYLRAEAAQRPWNADRLRADARERLGAHPDQLDMLVDSAAPDRFADLLRVFGAAAGRRCKAFPNAERRVRRALAIGLGKQN 5FTG Chain:A ((3-372)) -QPEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG---------EAMVLESVMFAILAERSLG-PKLYGIFP--QGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEP------KWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQ---------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1091 10451 9.58 37.06 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 9.58 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.273

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: