Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESTDEPGDTVDTELFTRVVRHGLPDV-TVETVTPGGPSWNPVTEVARVGFADRDPVYCKAAPGDHEGDLRAEAGTLDYVGATLRVRVPSVVRVT-TEPVAALLTEPVAGRAVADEWFETTPERRATLAERLGRTLATVHTERFD-RAGEITGG---DPDGLALDHAPWPEVLRA-GVTETQRRAPT-DRFDPECARLLDAIDDSRDRLADPPARLLHRDPATPNCFDTGDDRLTLLDWGNSVVGDPVCDLVRAREQVLAPSREPAPDRLVAPLRRGYRAVAGGVPSAADREPVYEAAITLSTAGFVDRLAEWREESVAELTAWFRAELDGRLSAVE 3JR1 Chain:A ((14-288)) -----NLYFQGMWKSISQVLAEQFGAYYFIKHKEKL--YSGEMNEIWLINDE-VQTVFVKINERSYRSMFRAEADQLALLAKTNSINVPLVYGIGNSQGHSFLLLEALNKSKNK-----------QSSFTIFAEKIAQLHQIQGPDKYGLDFDTWLGPIYQPNDWQTSWAKFFSENRIGWQLQICKEKGLIFGNIDLIVQIVADTLSK-HNPKPSILHGNLWIENCIQVDDK-IFVCNP-ACYWGDRECDIAFSSL--FE--------PFPTNFYQRYNEIYPLEEGYLERKLIYQLYYLLNFSYRY------------------------------

General information: TITO was launched using: RESULT: Template: 3JR1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1297 18072 13.93 67.68 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 13.93 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.191

(partial model without unconserved sides chains): PDB file : Tito_3JR1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3jr1-query.scw PDB file : Tito_Scwrl_3JR1.pdb: