Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAANPPRPSRSLQGKVAIVTGAGSRGETIGNGRAIALLLAEDGAKVVCVDREEALAQRTAELVLAENEGASALAVAADVSSAEECARVVDAALRLFGRVDVLVNGVGVLGPPGTAVDVDAARWARGLEVNVTSMMLMAKYAVPAMRDNEPEAGVRGSIVNLGSVAGLRGGTPSLLYPTSKCAVVNMTRAMAAHHAKDGVRVNCVCPGMLYTPMVYDAEGGMSAEVREARRRRSLLQTEGNAWDCAGPVRFLAGGEARWITGTVLTVDAGATCALAVSMPDGE 1FMC Chain:A ((1-255)) MFNSDN----LRLDGKCAIITGAGA-----GIGKEIAITFATAGASVVVSDINADAANHVVDEIQQ--LGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPF--DMPMADFRRAYELNVFSFFHLSQLVAPEMEKNG-----GGVILTITSMAAENKNINMTSYASSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALK---SVITPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN-------

General information: TITO was launched using: RESULT: Template: 1FMC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1436 -158609 -110.45 -621.99 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -110.45 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_1FMC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1fmc-query.scw PDB file : Tito_Scwrl_1FMC.pdb: