Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRLDGKVIILTAAAQGIGQAAALAFAREGAKVIATDINESKLQELEK-------YPGIQTRVLDVTKKKQIDQFANE---VERLDVLFNVAGFVHHGTVLDCEEKDWDFSMNLNVRSMYLMIKAFLPKMLAQKSGNIINMSSVASSVKGVVNRCVYSTTKAAVIGLTKSVAADFIQQGIRCNCVCPGTVDTPSLQERIQARGNPEEARNDFLKRQKTGRFATAEEIAMLCVYLASDESAYVTGNPVIIDGGWSL 2Z1N Chain:B ((3-260)) -LGIQGKLAVVTAGSSGLGFASALELARNGARLLLFSRNREKLEAAASRIASLVSGAQVDIVAGDIREPGDIDRLFEKARDLGGADILVYSTGGPRPGRFMELGVEDWDESYRLLARSAVWVGRRAAEQMVEKGWGRMVYIGSVTLL-RPWQDLALSNIMRLPVIGVVRTLALELAPHGVTVNAVLPSLILTDRVRSL------------SMASRIPMGRVGKPEELASVVAFLASEKASFITGAVIPVDGGAHI

General information: TITO was launched using: RESULT: Template: 2Z1N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1284 -17058 -13.29 -73.84 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -13.29 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_2Z1N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2z1n-query.scw PDB file : Tito_Scwrl_2Z1N.pdb: