Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNQNVLLNISG---------VKFVLRIPNAVNLSLINREYEAFNNAQAYRAGLNVETPVLDAKSGVKLTRYLENSNTLSQIQLNEQSCLSQVVNNLYRLHNSEFVFRNVF-SVFDEFRQYFSLLENKSAFYQ--AD----SRMDKLSAVFWQFEEI--NKEVILRPCHNDLVPENMLLQD-------DRLFFIDWEYSGLNDPLFDIATIIEEAHL------------------SKEAADFLLETYCNQTNKYH--------KTEFQIAHKRLKIHRFCQNVLWFLWTKVKEEHGE---NFGDYALKRLDAAFKLLEELP 5FTG Chain:A ((42-376)) -SNMLFQCSLPDTTATLGDEPRKVLLRLYG------EAMVLESVMFAILAERSLGPKLYGIF--PQGRLEQFIPSRR-LDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKL-

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 890 3593 4.04 14.04 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 4.04 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: