Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPSHLTHIWRLLKWGRTLARHGALTGIERDPLTPTPVRRLVRVARLGARVPRQPRYADAFQSIGPAAIKLGQTLATRPDLVGEDAANDLLRLQDALPPVPFATIRAQMEQSFGR-PLETL---YSRFDE-APVGAASIAQVHRAVTT-DGRDVAVKVIRPGVIDQFNRDIQTYEWAAAHVEMLGGEVARLRPRLVIANMKRWTARELDLRREAASASEL-GEAMEAMPGYRVPAIDWDRTTGKVMTMEWIDGVKISDRDALIA--AGHDVKEIAARLVNAFLRQAIAEGFFHADMHQGNLFVTANGDIVAIDFGIMGRIDRRARMWLAEILYGLITGNYRRVAEIHFE------AQYVPGHHNVEEFATALRAVGEPMRGKPVRELSVGGMLDGLFAITRDFDMQTQPHLLLLQKTMVMVEGVATALDPDINLWETSGPYVKEWLRAELGPEAKAADAL-IENVRILQRLPGLVKRIEEAFPEKGGAPPPPPLTEVRLIRVGSGWRYMLVALLAGLAGAGAMAAATTFL 2Y7J Chain:A ((82-362)) ----------------------------------------------------------------------------------------------------------------ELPDWAAAKEFYQKYDPKDVIGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEV------RE--------------------------ATRRETHILRQVAGH-----PHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVALSEKETRSIMRSLLEA-VSFLHANNIVHRDLKPENILLDDNMQIRLSDFGFSCHLEPGEKL-------RELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVILFTLLAGS--PPFWHRRQILMLRMIME--GQYQFSS---------------------PEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPF------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Y7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1212 -10327 -8.52 -38.97 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -8.52 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.166

(partial model without unconserved sides chains): PDB file : Tito_2Y7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2y7j-query.scw PDB file : Tito_Scwrl_2Y7J.pdb: