Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIQQEIFSILGI---KGPVLLGEGSESQTYDYT----ADKVVKIYKGPVEQDNLDHLCSIYTWLSTHHLPFAVPEIYAIESH-EGTVY----LIEKKLAGRPISAVYAQLQMYERQRLLSNYLEAVRHLQGVVLPTQPYGSIFHAPGPSPVPYQSWRKFLRETAPLKLPQTQDDLRQEGIDVARLLEKFFQDIEN-APDTPEKNFVHGDYFFGNVLVDDELEISAVLDVSWWSVVGDHLMDIAGAVMFLDLYEYVTPQEIASLTQQALALYGPEI---LTAIRIYGVYYSLLLSDCKL--LDPAAYSWSIKRLREYMQ 5IGI Chain:A ((12-298)) ---LYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNR-LPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV---VPQDSEVFAESFATALAALHAVPISAAVDAGMLI------RTPTQARQKVADDVDRVRR----EFV----VNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSE-ARVDDPAIDMAAHLMVFG------EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLA----

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1238 -35790 -28.91 -133.54 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -28.91 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.300

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: