Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MDD---LDKPIAYGRTAEIFAWGEN-----QVLKLFHAWVGFDAVEYEYRIARTMQSCGLPVPQVGEIIEYKGRYGITCQRIKGISMTESLAR--APWKILRYARRMAELHARVHAVTHPTDIPPLNPKMARKILQAQSLPARLREKALTRLEKLPEGHDLCHGDFHPDNILLTPQGE------VILDWMDAGT-------------------------------G---NPLADLARTSIILK----GSIESGQIRHPLLKRLAHLFHRAYLAQ-YFSLRPGGESEYALWLPIIAAARLSEN--ISELEGWLVAQAER-GLS---------- 1CSN Chain:? ((6-298)) NVVGVHYKVGRRIGEGSFGVIFEGTNLLNNQQVAIKFEPRRSDAPQLRDEYRTYKLLAGCT-GIPNVYYFGQEGLHNVLVIDLL-GPSLEDLLDLCGRKFSVKTVAMAAKQMLARVQSIHEKS---------------------------------------LVYRDIKPDNFLIGRPNSKNANMIYVVDFGMVKFYRDPVTKQHIPYREKKNLSGTARYMSINTHLGREQSRRDDLEALGHVFMYFLRGSLPWQGLKAATNKQKYERIGEKKQSTPLRELCAGFPEEFYKYMHYARNLAFDATPDYDYLQGLFSKVLERLNTTEDENFDWNLL

General information: TITO was launched using: RESULT: Template: 1CSN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1015 -6349 -6.26 -28.47 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -6.26 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.301

(partial model without unconserved sides chains): PDB file : Tito_1CSN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1csn-query.scw PDB file : Tito_Scwrl_1CSN.pdb: