Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEVREYGKKSQPKIRYSQFDAEDIRKELLERYGMEMPVECRLFDYG-MNDIYRIKTK-KETYFLRISLAGLHTKKDYEEEMHILNILIQN-GIKAAAPVKMRGMPGRQEDYVWELHAPEGIRYAMLFTEAPNSPS-D-----DKESSACNLGSMLAGIHNISDREKFQVSRPPIDMCQLVEKPLSNIRPFLADREQDFSFLKESAEKLAGYIKGKLPETAPVYGFCHGDVHSGNVFFA-G-DKPTLFDFDCMGYGWRSYDICVYAWNETFLDENYIEGSVWKKYLEGYESVRPLSAE-EKETIPAFAALRQLWLMGLHADVMDRNAGCCWYQDDYFDSQIKMFRLWLDRCSL 5IGI Chain:A ((6-280)) ------------------TADTSQLYA-LAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEV-SAKVEPEARVLAMLKNRLPFAVPDWR-----VANAE-LV-AYP---MLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREF----VVNDKRLHRWQRWLDD-DSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG--------EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALF-----------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1180 -18151 -15.38 -69.28 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -15.38 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: