Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------MNLEQDIRAILRTEIKKHLKFKDVQCELNEIRSKNGVYVYRIITTSTSYVIKYFEKNEFRRELDYYNLLIELEIPTIPVISMTD-QSIILEDIEQ-------SETYRLGFESDLENE-------NVARGLVAWYTQFHKKGRI-YLKQNPEQKASL---YNE--------------------YNELTKNVITDIMTKTG-----TNHYKYWHILIN----------HFDDLIAQSK----SYTSTLTYNDFYYTNFIVSKDESEVIMFDYNFLGVGYRYSDVRNIVSSLKGNAK----------------SLFLACYPQCNEGEVILDDVLADLFVLKEAYKRDQF----PSWAK-----------IPVEQLDNGVFEQKVLKLKHYFKFD 1MXA Chain:? ((1-383)) AKHLFTSESVSEGHPDKI-ADQISDAVLDAILEQDPKAR-VACET--YVKTGMVLVGGEITTSA-WVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQS---PDRADPLEQGAGDQGLMFGYATN-ETDVLMPAPITYAHRLVQRQAEVRKNGTLPWLR--PDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLTSATKFF---INPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFS----GKDPSKV-----DRSAAYAARYVAKNIVAA--GLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTD------KAQLLRDAAGLK

General information: TITO was launched using: RESULT: Template: 1MXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1457 35608 24.44 131.39 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 24.44 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.259

(partial model without unconserved sides chains): PDB file : Tito_1MXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1mxa-query.scw PDB file : Tito_Scwrl_1MXA.pdb: