Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKVEAILREMGDQSALNEVTPVSGGDISRAFQAKTANGVYFIKINDKAP--EDFFLKEAEGLNLLRQAGALPVPQVYYVSPPS---------AEDGYIIMEWVKG-ES-----APNTESRLGHGLALLHQHTHTHYGLAENNYIGRLPQPNRWEKSWTRFLREH-RLGFQAKLAEKRGRLPAGRKTRLEKLLDRLEQWV----PDYRQPVLLHGDLWSGNWLAGPR---GEPYLIDPA-VFYGEREFELAFTELFGG-------FSPRFYAAYRDVQPLSDNYEEVRPLYQLYYLLVHLNLFGESYGPSVDRILTYYAG 6EF6 Chain:A ((43-312)) -----------------NASLRLL-NLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQR-WTRPA--GFGR----FSW--DWEHCLGDTPRWGRWQDAEG----VGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRL-----------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 960 7142 7.44 30.52 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 7.44 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: