Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFARDALSTAVETACDPDPAVDTISTIEAGNN-HTYRVTLADGTDLFLKVGTR--FPDAFPAEPATMRRVRAE--TDLPVPRVHGTGTDPLEYPYAVYEYVPDCEAEWA-G-DLSHEAAERLCREAGRHLCELHQITFPQFGRIGLDTDASADDTLVADAPDADALTVVDPTPFDDLLRQSLDRQVTELADSPFDTRRDSLEAFGTDLIDRSDFADVTPALVHGDYRLGNLCVDPSAEQITAAVLDWELPTALDPLWDAVMAQSLLTAGHRLDPDSRQTLRRAFWEAYGTSTAD---TPRRRCYELLARIRLARHLDTECRGLSEAARRARITDHHEAFDALLVDR 5IQC Chain:A ((10-281)) --TNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVN----NEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISD-ELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNK-Q-----------------NVLEEYILLRETIY----NDLT----DIEKDYIESFMERLNA-TTVFEGKKCLCHNDFSCNHLLLDG--NNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEI------GTNFGEDILRMY-GNIDIEKAKEYQDIVEEYYPIETIVY-------------------------------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1164 15016 12.90 57.31 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 12.90 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.310

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: