Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRSVHDALASHPV--ETTVHRRLHDVRPHEVFEVTYDGQRVVCKVAQHPL----GDP-ELEARILQFVAAETPVPVPPIMAVG----------SDHFVAGWCRDLPADP-TVDETRSRAMGAGLARLHESASERFDAPGHLEAENGSLALDADDRWSDTLVAFLDERRR-FLE-PRGYGDVATEVLGLVEAFP---EAF-DDVGDPTLLHGNALPDHVGIDRSADPARVTRVIDFEHALVGPPAFDLLRSIGPTYGPP---------GATHDPDGRAAFLEGYAT--VRPLPADLEETFRPLGVVNGVAYLRALHLQRGDRDAPQSVARRARGLAEHVLEAASEVRSALRSE 4OCV Chain:A ((25-318)) -NEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKA-QGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGF-DANQLTE-T-----IAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDAT--TGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFS-TELFRAYTEGFVGELRDSITAREAELLPFSGNL-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1059 -4241 -4.00 -16.37 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -4.00 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: