Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERIKINSITIDEAKKHAVFVVKKELCCNVNKVQYLGGGSFGYIYKVDIDRPPYTVVMKACRCDNMCEREAFELNLLAKDSLIQIPKVYFTYLKDDIVSIDYICMEYVEGKDCFTDFSKLFLSKAKKRAFADKITSAMYAWHSKTNDKF---GLVQNA--N-YDEWLDYYKPFAFDILQKARNMVDRGELESYFLKVMELAWNNFDYIFS-EKVEHASLIHGDLNVMNILADDKLNPIAIIDPLESKWADREYDLFQLKNLT---GNAFGLYEMYKSKYPTSKNCDLKVAFYAMFHEIYCYIRSGRRTKINHLRCLLNLKIELNNVSIHS 3W0O Chain:A ((9-277)) ---------------TSVEKFLIEKFG-SVSDLMQLSEGEESRAFSFDVGGR--GYVLRVNSCADGFYKDRYVYRHFAS-AALPIPEVLDIGEFS--ESLTYCISRRAQGVTLQ----D--LPETELPAVLQPVAEVMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIA---DPHVYHWQT--VMDDTVSASVAQALDELMLW-AEDCPEVRHLVHADFGSNNVLTDNGRI-TAVIDWSEAMFGDPLYEVANIFFWRPWLACMEQQARYFERRHPEL-AGSPRLRAYMLRIGLDQLYQS--------------------------

General information: TITO was launched using: RESULT: Template: 3W0O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1163 -74795 -64.31 -288.78 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -64.31 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.347

(partial model without unconserved sides chains): PDB file : Tito_3W0O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3w0o-query.scw PDB file : Tito_Scwrl_3W0O.pdb: