Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHDKAFTFQTLRPDTIIDGLFD-LGMR------VDSGLTPL-NSYENRVYQFQDEE--------RHRYVVKFYRPERWS-AEQILEEHQFALQLVEDEVPVAAPLLFNDSTLHQHQGFYFAVFPSLGGRQFEAD---NLDQMEWVGRYLGRLHQTGRKQRFTARPEIG-VQEYLLEPRQVFEQA----TLIPSGLKADFLKATDKLIAAVMEQWHGRGNTLRLHGDCHAGNILWR-D-------GPLFVDLDDARTGPAIQDLWMLLNGDKAE-----------------QRMQLETIVEAYEEFSPF-------------NSDEIALIEPLRAMRLVYYLAWLLRRWDDPAFPVNFPWLTGEDYWRGQTSTFLEQVKVLQEPPLQLTPMY 5FUT Chain:A ((8-376)) -----------TRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----GAEAMVLESVMFAILAERSLG-PKLYGIF---------PQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLES---TPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKIS--SIEF---GYMDYAQARFDAYFHQKRKL-----------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1084 -24661 -22.75 -87.14 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -22.75 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: