Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFQLDKDWRLQPI-KGATGQTYMGIKE--TEKVFIKRNTSP------------LLAALSKEGLAPKMIWTKRTANGDVLTAQEWLEGRLLHADE---IGKRNDVIDVLYQLHHSNLLKDMLKK-------IGGEVCTPLTMLKEYKRQLPKELK---------------RNSYLKEVITYLYNHLPEYPETSYVAVHGDVNHRNWLVSN--NYLYLVDWDSVMVADPAVDLGMILSHYV----------PRSGWNQWLLSYGLIPN----ESTVQRIYWYGLFSFLQEIVRHHQEGERRAMTAEILQLKRMFSI 2QG7 Chain:E ((110-432)) -------SLEFQIING-ITNILIKVKDMSKQAKYLIRLYGPKTDEIINREREKKISCILYNKNIAKKIYVFF--TNG---RIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCK-RENSPIVLCHCDLLSSNIINTVGGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMH----------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 884 -4179 -4.73 -18.25 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain E : 0.65 3D Compatibility (PKB) : -4.73 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: